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pkh26 red, fluorescent membrane linker dye  (Millipore)


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    Millipore pkh26 red, fluorescent membrane linker dye
    Pkh26 Red, Fluorescent Membrane Linker Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pkh26+(red)+membrane+dye/pkh26/10__1016_slash_j__cej__2024__155336-61-9-37
    Average 90 stars, based on 1 article reviews
    pkh26 red, fluorescent membrane linker dye - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect.
    Article Snippet: Neuroblastoma (NB) is the most common extracranial solid tumor in the pediatric population with a high degree of heterogeneity in clinical outcomes.. Upregulation of the tumor suppressor miR-204 in neuroblastoma is associated with good prognosis.. Although miR-204 has been recognized as a potential therapeutic candidate, its delivery is unavailable.

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders
    Article Snippet: .. Freshly isolated mouse thymocytes were labeled with the red fluorescent cell membrane dye PKH26 (Sigma-Aldrich) following the manufacturer's instructions. .. Subsequently, cell apoptosis was induced by 1 μM dexamethasone (Sigma-Aldrich) overnight and labeled with PKH26.

    Article Title: Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression
    Article Snippet: Furthermore, the size and quantitation of exosomes were analyzed using a NanoSight NS300 instrument (Malvern Instruments) equipped with NTA 3.0 analytical software (Malvern Instruments). .. Purified exosomes isolated from the culture medium were collected and labeled with PKH26 Red Fluorescent membrane linker dye (Sigma-Aldrich) according to the manufacturer's instructions. .. Then, labeled exosome pellets were resuspended and added to the unstained macrophages for exosomes uptake studies.

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Article Title: Human Tumor–Associated Macrophages and Neutrophils Regulate Antitumor Antibody Efficacy through Lethal and Sublethal Trogocytosis
    Article Snippet: The data were analyzed, and tumor cell growth curves were generated using GraphPad Prism using GraphPad Prism 9 software. .. Daudi and A431 tumor cells were labeled with cell membrane dye PKH67 Green Fluorescent Cell Linker (Sigma) and FcR + effectors were labeled with cell membrane dye PKH26 Red Fluorescent Cell Linker (Sigma), according to the manufacturer's protocol. .. The PKH-labeled effectors and targets were plated into a poly-D-lysine-coated 8-well chamber μ-slide (Ibidi) in AIMV cell culture media with AlbuMAX Supplement (Thermo Fisher Scientific).

    Article Title: Hypoxic lung adenocarcinoma-derived exosomal miR-1290 induces M2 macrophage polarization by targeting SOCS3.
    Article Snippet: For exosome staining, a total of 3% phosphotungstic acid was applied to the grid for 1 min. After drying, TEM was used to observe the morphology of the stained exosomes. .. Purified exosomes were labeled with PKH26 red fluorescent membrane linker dye (Sigma– Aldrich) according to the manufacturer's instructions. .. After removal of excess dye, the labeled exosome pellets were resuspended and added to the cultured macrophages for the studies of exosome uptake.

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells.
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Labeling:

    Article Title: Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect.
    Article Snippet: Neuroblastoma (NB) is the most common extracranial solid tumor in the pediatric population with a high degree of heterogeneity in clinical outcomes.. Upregulation of the tumor suppressor miR-204 in neuroblastoma is associated with good prognosis.. Although miR-204 has been recognized as a potential therapeutic candidate, its delivery is unavailable.

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders
    Article Snippet: .. Freshly isolated mouse thymocytes were labeled with the red fluorescent cell membrane dye PKH26 (Sigma-Aldrich) following the manufacturer's instructions. .. Subsequently, cell apoptosis was induced by 1 μM dexamethasone (Sigma-Aldrich) overnight and labeled with PKH26.

    Article Title: Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression
    Article Snippet: Furthermore, the size and quantitation of exosomes were analyzed using a NanoSight NS300 instrument (Malvern Instruments) equipped with NTA 3.0 analytical software (Malvern Instruments). .. Purified exosomes isolated from the culture medium were collected and labeled with PKH26 Red Fluorescent membrane linker dye (Sigma-Aldrich) according to the manufacturer's instructions. .. Then, labeled exosome pellets were resuspended and added to the unstained macrophages for exosomes uptake studies.

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Article Title: Human Tumor–Associated Macrophages and Neutrophils Regulate Antitumor Antibody Efficacy through Lethal and Sublethal Trogocytosis
    Article Snippet: The data were analyzed, and tumor cell growth curves were generated using GraphPad Prism using GraphPad Prism 9 software. .. Daudi and A431 tumor cells were labeled with cell membrane dye PKH67 Green Fluorescent Cell Linker (Sigma) and FcR + effectors were labeled with cell membrane dye PKH26 Red Fluorescent Cell Linker (Sigma), according to the manufacturer's protocol. .. The PKH-labeled effectors and targets were plated into a poly-D-lysine-coated 8-well chamber μ-slide (Ibidi) in AIMV cell culture media with AlbuMAX Supplement (Thermo Fisher Scientific).

    Article Title: Hypoxic lung adenocarcinoma-derived exosomal miR-1290 induces M2 macrophage polarization by targeting SOCS3.
    Article Snippet: For exosome staining, a total of 3% phosphotungstic acid was applied to the grid for 1 min. After drying, TEM was used to observe the morphology of the stained exosomes. .. Purified exosomes were labeled with PKH26 red fluorescent membrane linker dye (Sigma– Aldrich) according to the manufacturer's instructions. .. After removal of excess dye, the labeled exosome pellets were resuspended and added to the cultured macrophages for the studies of exosome uptake.

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells.
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Incubation:

    Article Title: Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect.
    Article Snippet: Neuroblastoma (NB) is the most common extracranial solid tumor in the pediatric population with a high degree of heterogeneity in clinical outcomes.. Upregulation of the tumor suppressor miR-204 in neuroblastoma is associated with good prognosis.. Although miR-204 has been recognized as a potential therapeutic candidate, its delivery is unavailable.

    Staining:

    Article Title: Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect.
    Article Snippet: Neuroblastoma (NB) is the most common extracranial solid tumor in the pediatric population with a high degree of heterogeneity in clinical outcomes.. Upregulation of the tumor suppressor miR-204 in neuroblastoma is associated with good prognosis.. Although miR-204 has been recognized as a potential therapeutic candidate, its delivery is unavailable.

    Isolation:

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders
    Article Snippet: .. Freshly isolated mouse thymocytes were labeled with the red fluorescent cell membrane dye PKH26 (Sigma-Aldrich) following the manufacturer's instructions. .. Subsequently, cell apoptosis was induced by 1 μM dexamethasone (Sigma-Aldrich) overnight and labeled with PKH26.

    Article Title: Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression
    Article Snippet: Furthermore, the size and quantitation of exosomes were analyzed using a NanoSight NS300 instrument (Malvern Instruments) equipped with NTA 3.0 analytical software (Malvern Instruments). .. Purified exosomes isolated from the culture medium were collected and labeled with PKH26 Red Fluorescent membrane linker dye (Sigma-Aldrich) according to the manufacturer's instructions. .. Then, labeled exosome pellets were resuspended and added to the unstained macrophages for exosomes uptake studies.

    other:

    Article Title: Cancer Cell Membrane Wrapped Nanoparticles for the Delivery of a Bcl-2 Inhibitor to Triple-Negative Breast Cancer.
    Article Snippet: Overexpression of the antiapoptotic protein B-cell lymphoma 2 (Bcl-2) is correlated with poor survival outcomes in triple-negative breast cancer (TNBC), making Bcl-2 inhibition a promising strategy to treat this aggressive disease.. Unfortunately, Bcl-2 inhibitors developed to date have limited clinical success against solid tumors, owing to poor bioavailability, insufficient tumor delivery, and off-target toxicity.. To circumvent these problems, we loaded the Bcl-2 inhibitor ABT-737 in poly(lacticco-glycolic acid) (PLGA) nanoparticles (NPs) that were wrapped with phospholipid membranes derived from 4T1 murine mammary cancer cells, which mimic the growth and metastasis of human TNBC.

    Purification:

    Article Title: Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression
    Article Snippet: Furthermore, the size and quantitation of exosomes were analyzed using a NanoSight NS300 instrument (Malvern Instruments) equipped with NTA 3.0 analytical software (Malvern Instruments). .. Purified exosomes isolated from the culture medium were collected and labeled with PKH26 Red Fluorescent membrane linker dye (Sigma-Aldrich) according to the manufacturer's instructions. .. Then, labeled exosome pellets were resuspended and added to the unstained macrophages for exosomes uptake studies.

    Article Title: Hypoxic lung adenocarcinoma-derived exosomal miR-1290 induces M2 macrophage polarization by targeting SOCS3.
    Article Snippet: For exosome staining, a total of 3% phosphotungstic acid was applied to the grid for 1 min. After drying, TEM was used to observe the morphology of the stained exosomes. .. Purified exosomes were labeled with PKH26 red fluorescent membrane linker dye (Sigma– Aldrich) according to the manufacturer's instructions. .. After removal of excess dye, the labeled exosome pellets were resuspended and added to the cultured macrophages for the studies of exosome uptake.

    In Vivo:

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells.
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Injection:

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..

    Article Title: Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells.
    Article Snippet: .. For the purpose of in vivo tracking, both MDSPCs and BM-MSCs were labeled with the red fluorescent membrane dye PKH26, following the manufacturer’s instructions provided by Sigma-Aldrich (Burlington, MA, USA), immediately before their injection. ..



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    HCC cells-derived exosomes promote M2 polarization of macrophages. (A) Transmission electron micrograph of HCC cell-derived exosomes and human normal liver cell line (MIHA)-derived exosomes. Scale bar: 200 nm. (B) Exosomes released by different HCC cells or MIHA cells were detected by NanoSight particle tracking analysis. (C) Exosome markers Alix and CD63 proteins were detected by western blot assay. (D) Internalization of <t>PKH26-labeled</t> exosomes (red) by macrophages examined by laser scanning confocal microscope. Scale bar: 50 μm. (E) Relative gene expression of M1 markers (CD86, INOS, HLA-DR) and M2 markers (CD206, ARG1, CD163) of macrophages treated with MHCC97H-exo, MIHA-exo (100 mg/mL), or control (PBS, LPS and IL4). (F) Flow cytometry was used to detect the expression of M2 marker (CD206) in macrophages treated with MIHA-exo, HepG2-exo, MHCC97H-exo, (100 mg/mL), or PBS. (G) Western blot analysis was used to detect M2 markers (CD206, ARG1) and M1 markers (CD86, INOS).
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    Analysis of plasma MVs by Zetasizer Nano ZS showing a large population represented by one peak at size range of ~ 100–1000 nm, specific to MVs (Y-axis represents the number of MVs and X-axis the size of MVs) ( A ). Examination of plasma MVs by fluorescence microscopy using <t>PKH26</t> staining technique ( B ), and electron microscopy using negative staining technique ( C )
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    Image Search Results


    HCC cells-derived exosomes promote M2 polarization of macrophages. (A) Transmission electron micrograph of HCC cell-derived exosomes and human normal liver cell line (MIHA)-derived exosomes. Scale bar: 200 nm. (B) Exosomes released by different HCC cells or MIHA cells were detected by NanoSight particle tracking analysis. (C) Exosome markers Alix and CD63 proteins were detected by western blot assay. (D) Internalization of PKH26-labeled exosomes (red) by macrophages examined by laser scanning confocal microscope. Scale bar: 50 μm. (E) Relative gene expression of M1 markers (CD86, INOS, HLA-DR) and M2 markers (CD206, ARG1, CD163) of macrophages treated with MHCC97H-exo, MIHA-exo (100 mg/mL), or control (PBS, LPS and IL4). (F) Flow cytometry was used to detect the expression of M2 marker (CD206) in macrophages treated with MIHA-exo, HepG2-exo, MHCC97H-exo, (100 mg/mL), or PBS. (G) Western blot analysis was used to detect M2 markers (CD206, ARG1) and M1 markers (CD86, INOS).

    Journal: International Journal of Biological Sciences

    Article Title: Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression

    doi: 10.7150/ijbs.94440

    Figure Lengend Snippet: HCC cells-derived exosomes promote M2 polarization of macrophages. (A) Transmission electron micrograph of HCC cell-derived exosomes and human normal liver cell line (MIHA)-derived exosomes. Scale bar: 200 nm. (B) Exosomes released by different HCC cells or MIHA cells were detected by NanoSight particle tracking analysis. (C) Exosome markers Alix and CD63 proteins were detected by western blot assay. (D) Internalization of PKH26-labeled exosomes (red) by macrophages examined by laser scanning confocal microscope. Scale bar: 50 μm. (E) Relative gene expression of M1 markers (CD86, INOS, HLA-DR) and M2 markers (CD206, ARG1, CD163) of macrophages treated with MHCC97H-exo, MIHA-exo (100 mg/mL), or control (PBS, LPS and IL4). (F) Flow cytometry was used to detect the expression of M2 marker (CD206) in macrophages treated with MIHA-exo, HepG2-exo, MHCC97H-exo, (100 mg/mL), or PBS. (G) Western blot analysis was used to detect M2 markers (CD206, ARG1) and M1 markers (CD86, INOS).

    Article Snippet: Purified exosomes isolated from the culture medium were collected and labeled with PKH26 Red Fluorescent membrane linker dye (Sigma-Aldrich) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Transmission Assay, Western Blot, Labeling, Microscopy, Expressing, Control, Flow Cytometry, Marker

    Analysis of plasma MVs by Zetasizer Nano ZS showing a large population represented by one peak at size range of ~ 100–1000 nm, specific to MVs (Y-axis represents the number of MVs and X-axis the size of MVs) ( A ). Examination of plasma MVs by fluorescence microscopy using PKH26 staining technique ( B ), and electron microscopy using negative staining technique ( C )

    Journal: Cardiovascular Diabetology

    Article Title: Microvesicle-associated and circulating microRNAs in diabetic dyslipidemia: miR-218, miR-132, miR-143, and miR-21, miR-122, miR-155 have biomarker potential

    doi: 10.1186/s12933-023-01988-0

    Figure Lengend Snippet: Analysis of plasma MVs by Zetasizer Nano ZS showing a large population represented by one peak at size range of ~ 100–1000 nm, specific to MVs (Y-axis represents the number of MVs and X-axis the size of MVs) ( A ). Examination of plasma MVs by fluorescence microscopy using PKH26 staining technique ( B ), and electron microscopy using negative staining technique ( C )

    Article Snippet: For fluorescence microscopy analysis, MVs were stained with red fluorescent membrane linker dye PKH26 (Invitrogen, Waltham, MA, United States) according to the manufacturer’s instructions and the protocol described by [ ].

    Techniques: Fluorescence, Microscopy, Staining, Electron Microscopy, Negative Staining